[ANAL-20]Micro flow reactors with an integrated optical trace oxygen sensor

The presented micro flow reactors with integrated optical trace oxygen sensor was fabricated in a mild and non-cleanroom environment. It was sensitive to the amount of dissolved oxygen in tetrahydrofuran. Under the luminescence microscope, it had KSV of 0.027 % v/v-1 and limit of detection of 0.14 % v/v oxygen in tetrahydrofuran. It will be used to monitor the concentration of oxygen in chemical syntheses.

Continue reading

[ANAL-19]Continuous preparative separation of nanoparticles on a free-flow electrophoretic microchip

In this work, a low-cost, straightforward, and fast fabrication method by using poly(ethylene glycol) diacrylate (PEG-DA) was presented to produce a micro free-flow electrophoresis (µFFE) device; the photolithography of PEG-DA laminated between two glass slides allows simultaneous bonding and structural development of the microfluidic layout. The most encountered issue of electrolytic bubbles intrusion in the separation chamber was successfully solved via integration of conductive hydrogel walls, which provides physical isolation while maintaining the electrical conductivity between the channels.

Continue reading

[ANAL-15]Mass spectrometry-based metabolomics investigation on two contrasting indica rice grains (Oryza sativa L.) under cadmium stress

Considering the toxicity of cadmium rice, many solutions for addressing the cadmium contamination in rice have been proposed. The selection and breeding of low-cadmium-accumulating rice varieties with high yields is one of the most economical and ecological method. This study utilized the metabolomics to investigate two contrasting indica rice grains under cadmium stress for the first time. Metabolic pathways related to carbohydrates, organic acids and amino acids gave identical and characteristic responses. The scientific approach-based biomarkers and characterization of metabolic pathways might be helpful for the selection of rice varieties with low-cadmium accumulation and shorten the cultivated time.

Continue reading

[ANAL-14]Preparation of frozen sections of multicellular tumor spheroids coated with ice for mass spectrometry imaging

A sectioning method based on ice-coated cell spheroids was established to prepare frozen sections of MCTS for MALDIMSI analysis. Compared to the traditional GAS method, the
developed method exhibited good visibility for small MCTS during cryosectioning, which could greatly reduce the time and cost for sample preparation. The optimized method was
successfully applied to observe the metabolite distribution in MCTS by using MALDI-MSI. The obtained results showed no signal suppression and delocalization. This method may also be applied to prepare frozen sections for other macroscopic biological samples such as pollen and zebrafish embryos.

Continue reading