[ANAL-08]The application of a real-ambient fine particulate matter exposure system on different animal models

To simulate real fine particulate matter (PM2.5) exposure, an ambient exposure system was installed in Taiyuan, China to study impacts of chronic PM2.5 exposure on Sirtuin3 knockout (Sirt3 KO) mice and aged mice. The case studies indicated that 15-week of real-ambient PM2.5 exposure induce inflammation and heart failure in Sirt3 KO mice. Meanwhile, aged mice exhibited apparent heart dysfunction involving increased heart rate and decreased blood pressure after the real-ambient PM2.5 exposure.

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[ANAL-07]Serum metabolic changes associated with dioxin exposure in a Chinese male cohort

In this study, serum samples of 215 male adults living in Shenzhen, China, including those workers of Baoan waste incineration power plant, Kuisheng electronics factory, and AVARY electronics factory, were recruited to quantify the concentrations of seventeen 2,3,7,8-substituted dioxins. Based on the exposure levels, metabolic profiles were investigated, and 20 potential biomarkers associated with inflammation, liver and cardiovascular diseases were identified. This study provides valuable information for understanding the potential toxicity associated with dioxin exposure.

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[ANAL-06]In vivo toxicity evaluation of new discovered disinfection byproducts – chlorinated methanesulfonic acids

The predicted toxicity of HMSAs by T.E.S.T.: Br2-MSA> Br-MSA> Cl2-MSA> Cl-MSA> BrCl-MSA. Based on the predicted LD50 values, 210 mg/kg and 420 mg/kg of Cl-MSA and Cl2-MSA mixture in 1:1 ratio were fed to two groups of ICR mice for 5 consecutive days by oral gavage. Urine samples of mice were collected right before and 8 hours after gavage daily. All mice were sacrificed after 5 days, with blood and organ tissues collected. Cl-MSA and Cl2-MSA content in urine and blood samples were analysed by LC-MS/MS.

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[ANAL-05]Effects of weathering on the sorption behavior and toxicity of polystyrene microplastics in multi-solute systems

More environmentally realistic particles of MPs with varied physicochemical properties were obtained from abiotic, biotic and their combination of both weathering processes in the laboratory. The major molecular-level interactions that control the sorption of environmental contaminants by MPs could be markedly affected by the alterations of surface properties upon various weathering processes. Consequently, the role of cosolutes with different properties could behave differently on the sorption uptake of primary solutes. Furthermore, it was observed that solute multilayers formed on the surfaces of PSMPs in the multi-solute system might induce a synergistic toxic effect to microalgae.

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[ANAL-04]Mapping and profiling of lipids metabolites alterations undergoing hydroxychloroquine in three-dimensional multicellular tumor spheroids using MALDI mass spectrometry imaging

We utilized MALDI-MSI in A549 lung cancer multicellular spheroids to reveal the spatial distribution of anticancer drug hydroxychloroquine (HCQ) and its effect on lipids. We have successfully observed the spatial variations of HCQ, which tended to penetrate in the inner core (proliferative zone) of treated spheroid. Lipidome results also demonstrated that HCQ treatment dramatically altered the composition of lipids in spheroids including the up regulation of phosphatidylcholine (PC), phosphatidylethanolamine (PE), which are potentially related to the drug resistance of cancer cells.

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[ANAL-03]Non-invasive samples analysis by laser ablation-inductively coupled plasma-mass

Human biomonitoring (HBM) using non-invasive sample types, such as hair and nails as biological samples has emerged in the past decade. The aim of the study was to optimize an analytical method for accurate determination of elemental contents in hair and nails samples by using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Seven elements (Mn, Cu, Zn, Sr, Y, Pb, and U) were included in the present study. The developed analytical method was applied in a pilot HBM study involving subject with hypothyroidism history. The preliminary results suggested that LA-ICP-MS using non-invasive samples posed a great potential in HBM study.

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[ANAL-02]Reliable and reusable whole polypropylene plastic microfluidic devices for a rapid, low-cost antimicrobial susceptibility test

Primarily caused by the misuse and abuse of antibiotics, antimicrobial resistance (AMR) is a major threat to modern medicine. The antimicrobial susceptibility test (AST) is a promising technique to help with the optimal use of antibiotics for reducing AMR. However, current phenotypic ASTs suffer from long turnaround time, while genotypic ASTs suffer from low reliability, and both are unaffordable for routine use. This work demonstrates a practical method to fabricate microfluidic chips entirely from polypropylene (PP) which providing a practical solution to improve current culture-based AST and benefiting the fight against AMR through helping doctors prescribe effective, narrow-spectrum antibiotics.

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[ANAL-01]Polydopamine patterned perfluorinated surfaces for bead-free digital immunoassays

We developed a bead-free digital ELISA approach using a PDA patterned perfluorinated substrate. By negative microcontact printing with improved resolution, we proposed a robust and simple method to create millions of microdroplets. We were able to capture single target proteins on each PDA spot without microbeads, greatly economizing the total cost of digital ELISA. Our platform achieved a over 270-fold higher sensitivity for human IL-6 detection compared with commercial ELISA kits, showing great potential in digital bioassays.

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