[BIO-29]Development of NAD tagSeq for identification and characterization of NAD+-capped RNA

A new strategy called NAD tagSeq was developed to identify NAD-capped RNA. The strategy mainly contains three steps. Firstly, an enzyme called ADPRC was used to modify the NAD cap with a clickable group. Then CuAAC reaction was performed to ligate tag RNA onto the NAD cap. Finally, the tagged RNAs underwent library preparation and Nanopore direct RNA sequencing. The strategy enables direct sequencing of NAD-capped RNA, thus facilitating further analysis of the RNA structure and capping ratio.

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[BIO-27]Study of benzofuroquinolinium derivatives as a new class of potent antibacterial agent targeting FtsZ

Drug-resistant bacterial infections have become one of the greatest threats to human health. In recent years, the bacterial cell division protein FtsZ has become an ideal new antibacterial target due to its widespread existence and conservative properties. Here, we carried out antibacterial activity evaluation and antibacterial mechanism research on cryptolepine derivatives. It was found that these derivatives showed strong antibacterial effects on both Gram-positive (including MRSA) and Gram-negative bacteria. The minimum inhibitory concentration (MIC) is as low as 0.25-32μg/mL. Mechanism studies have shown that these compounds can affect the polymerization activity and GTPase activity of purified FtsZ.

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[BIO-26]Interaction study of trans-membrane divisome protein complex FtsQBL by hydrogen-deuterium exchange mass spectrometer

The bacterial division depends on different protein complexes to achieve dramatic change. Divisome is one of them that responsible for the separation of membrane and peptidoglycan cell walls. This process is initiated by the Z-ring formation and more than 10 crucial protein assembly, the late division protein will then sequentially anchor onto this macrostructure1. FtsQ, FtsB and FtsL are important to connect two stages. Although some structural data and models have been proposed, the interplay between the trimeric protein complex FtsQBL remains unclear. This project is trying to get a better understanding of the whole trans-membrane protein complex interaction, using (HDXMS).

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[BIO-25]Nusbiarylin as a new class of antimicrobial compounds targeting the NusB-NusE protein interaction

Bacterial protein NusB and NusE are two essential transcription factors highly conserved and exclusively existing in bacteria. Drugs targeting NusB-NusE protein-protein interaction reduce the possibility of antibiotic resistance developed from comutations. We rationally designed and efficiently synthesized a new series of diaryl derivatives against the NusB-NusE interaction, confirmed by mechanistic studies. Some of these compounds exhibited excellent antibacterial activities against a panel of clinically isolated pathogens with minimum inhibitory concentration of 0.25-1 μg/mL, comparable to conventional antibiotics. Mechanism study suggested disruption of NusB localization. Preliminary pharmacokinetic studies demonstrated the potential druggability of our compounds.

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