[INORG-04]Neutral iridium(III) complexes tethered with a polyhedral oligomeric silsesquioxane nanocage for lipid droplet imaging and photodynamic therapy

The charge of a cellular reagent plays a significant role in the cell internalization and intracellular distribution, which is a prerequisite for theranostic applications. In this work, a new class of nonionic iridium(III) complexes tethered with a polyhedral oligomeric silsesquioxane (POSS) unit were synthesized and characterized for lipid droplet imaging and photodynamic therapy.

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[INORG-02] Utilization of rhenium(I) polypyridine complexes appended with a dinitrophenylsulfonyl moiety for bioimaging and photodynamic therapy

In this work, four rhenium(I) polypyridine complexes functionalized with a dinitrophenylsulfonyl (DNPS) moiety were designed for GSH-targeted imaging and photodynamic therapy. These complexes displayed weak emission due to efficient quenching by the DNPS unit. Upon reaction with intracellular GSH, the complexes showed substantial emission enhancement and time-dependent photocytotoxicity in HeLa cells.

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[BIO-03]Luminogenic bioorthogonal iridium(III) bis-tetrazine complexes for selective labeling and stapling of BCN-tagged peptides and cell imaging

Three novel luminogenic cyclometalated iridium(III) bis-tetrazine complexes were synthesized and characterized. The applications of these complexes as luminogenic double-clicking two-point binders for the derivatives of a strained alkyne, bicyclo[6.1.0]non-4-yne (BCN) were examined. Selective labeling and stapling of BCN-tagged peptides using the complexes were investigated by HPLC. Live cell imaging using the iridium hydrogel loaded with organic dyes BODIPY-Et and BODIPY-PEG40k was studied.

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[BIO-02]Modulation of emission and singlet oxygen photosensitisation in live cells utilizing bioorthogonal phosphorogenic probes and protein tag technology

The self-labeling protein SNAP-tag is commonly used to study the expression and functions of proteins of interest (POI) in live cells. It undergoes specific reactions with synthetic probes containing a benzylguanine (BG) moiety. Despite the development of many substrates for this protein tag, photofunctional substrates that serve as both fluorogenic probes and singlet oxygen (1O2) photosensitisers have not been reported. Herein, we designed a new class of phosphorogenic iridium(III) nitrone complexes for the two-step labeling of SNAP-tag to confer luminogenic properties and controllable 1O2 generation to the system.

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[BIO-01]Bioorthogonal phosphorogenic rhenium(I) polypyridine sydnone complexes for specific lysosome labeling

The development of new bioorthogonal reactions is necessary for a multitude of biological applications. In this work phosphorogenic rhenium(I) polypyridine sydnone complexes were designed for specific lysosomal labeling. The complexes were non‐emissive, but strain‐promoted sydnone‐alkyne cycloaddition reaction with the substrate BCN‐OH led to emission enhancement in the range of 8.8 to 17.3. Conjugation of the complexes with BCN‐BSA demonstrated emission enhancement factors as high as 38.9. Specific lysosomal labeling in live cells was achieved by using BCN‐morpholine and one of the rhenium(I) complexes.

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