[ANAL-29]Single particle ICP-MS combined with internal standardization for accurate characterization of polydisperse nanoparticles in complex matrices

A brief introduction of single particle inductively coupled plasma-mass spectrometry (sp-ICP-MS), the gaps between the real situation and researches and the objectives of this study is firstly presented. A simple diagram of the experimental procedures is then shown. The results of this study are presented in the following parts: Impact of matrix effect on mass determination by sp-ICP-MS, Stability of ionic Rh and CeO2 NPs after the addition of internal standard (IS) and Mass concentration determined by sp-ICP-MS after the addition of IS. Finally, a conclusion that matrix effect on sp-ICP-MS can be effectively corrected by internal standardization is drawn.

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[ANAL-15]Mass spectrometry-based metabolomics investigation on two contrasting indica rice grains (Oryza sativa L.) under cadmium stress

Considering the toxicity of cadmium rice, many solutions for addressing the cadmium contamination in rice have been proposed. The selection and breeding of low-cadmium-accumulating rice varieties with high yields is one of the most economical and ecological method. This study utilized the metabolomics to investigate two contrasting indica rice grains under cadmium stress for the first time. Metabolic pathways related to carbohydrates, organic acids and amino acids gave identical and characteristic responses. The scientific approach-based biomarkers and characterization of metabolic pathways might be helpful for the selection of rice varieties with low-cadmium accumulation and shorten the cultivated time.

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[ANAL-14]Preparation of frozen sections of multicellular tumor spheroids coated with ice for mass spectrometry imaging

A sectioning method based on ice-coated cell spheroids was established to prepare frozen sections of MCTS for MALDIMSI analysis. Compared to the traditional GAS method, the
developed method exhibited good visibility for small MCTS during cryosectioning, which could greatly reduce the time and cost for sample preparation. The optimized method was
successfully applied to observe the metabolite distribution in MCTS by using MALDI-MSI. The obtained results showed no signal suppression and delocalization. This method may also be applied to prepare frozen sections for other macroscopic biological samples such as pollen and zebrafish embryos.

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[ANAL-08]The application of a real-ambient fine particulate matter exposure system on different animal models

To simulate real fine particulate matter (PM2.5) exposure, an ambient exposure system was installed in Taiyuan, China to study impacts of chronic PM2.5 exposure on Sirtuin3 knockout (Sirt3 KO) mice and aged mice. The case studies indicated that 15-week of real-ambient PM2.5 exposure induce inflammation and heart failure in Sirt3 KO mice. Meanwhile, aged mice exhibited apparent heart dysfunction involving increased heart rate and decreased blood pressure after the real-ambient PM2.5 exposure.

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[ANAL-07]Serum metabolic changes associated with dioxin exposure in a Chinese male cohort

In this study, serum samples of 215 male adults living in Shenzhen, China, including those workers of Baoan waste incineration power plant, Kuisheng electronics factory, and AVARY electronics factory, were recruited to quantify the concentrations of seventeen 2,3,7,8-substituted dioxins. Based on the exposure levels, metabolic profiles were investigated, and 20 potential biomarkers associated with inflammation, liver and cardiovascular diseases were identified. This study provides valuable information for understanding the potential toxicity associated with dioxin exposure.

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